NSW Police Commissioner Mick Fuller said the Caledonian Hotel in Singleton would be closed until Tuesday after allegations of repeated breaches of public health orders.
Police have had multiple interactions with the 57-year-old licensee who allegedly refused to take steps to prevent unvaccinated people entering.
Only fully vaccinated people are allowed to enjoy greater freedoms like attending restaurants or pubs when the state started opening up last week.
“Police have attended the venue numerous times in the past week and issued the licensee three PINs and staff members three PINs for breaching the public health orders,” Mr Fuller said.
The pub has gained notoriety for its owner’s stance on vaccination.
Signs have been hung stating: “Jab or no jab – all welcome at the Cali. Free Australia.”